The Independent Validation Initiative is committed to improving the antibody and ELISA kit market. We provide independent, scientifically sound, and properly documented data.

Become a Validator will offer a full refund for approved validation proposals only. Refunds will be issued once submitted validation data has been reviewed and approved. Not all validation proposals will be approved, antibodies-online reserves the right to reject validation data if it does not meet our standards, or the agreed upon qualifications and criteria.  Participants agree to allow antibodies-online or its partners to view, edit, modify, and/or share any data submitted immediately and in perpetuity.

How does the Independent Validation Initiative work?

  • Share your validation data for one of our products
  • Help improve science
  • Get a full refund for validated products

Our team will contact you to discuss your future validations.

Participate in the Initiative

Adalberto Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

I can confirm that the process is truly independent.

Share your Validations and Improve Science

The Independent Validation Initiative is a project by

The Validation Process

The independent validation initiative is centered around core values of transparency, reproducibility, scientific integrity.

We are committed to improving the proteomics market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data.

We are the online market place for proteomics products. We sell over 1,600,000 products from more than 200 suppliers worldwide.

Order online, via email, phone, fax, or the purchase department of your institution.

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Become A Validator

It is easy to participate and you will get a full refund for the validated products:

  • Apply to participate in the independent validation initiative by proposing a validation experiment.
  • We review your proposal and approve it within 5 business days.
  • After approval, perform the validation experiment, and send us your validation data.
  • We provide a complete refund for the product you validated - pass or fail.

Sabine Schneider, Institute of Physiology,
University of Bern

I was actually surprised how uncomplicated the validation process is.

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Rules and Restrictions

David J Barnes, Institute of Musculoskeletal Sciences, NDORMS, University of Oxford

 I would recommend the independent
validation initiative and be happy to participate again in the future.

Veronika Neubrand, Departamento de Bioligía Celular e Inmunología, IPBLN, Granada

It was a very positive experience and I will participate in this program again.

Example Validations

Protein 1 (CREB1) antibody

Successfully validated (Western Blotting)
by Instituto de Parasitología y Biomedicina López-Neyra
No. #100101
Date 12/23/2016

Customer Validation ABIN969061

Western Blotting validation image for anti-CAMP Responsive Element Binding 

Primary mouse microglia were transfected with Creb1 siRNA and Creb1 was revealed using ABIN969061 as described in the protocol section (lanes 1, 2, and 3). Lysates from cells transfected with scrambled siRNA (lane 4) and untransfected cells (lane 5) served as negative controls.

Read the full report
Customer Validation ABIN1841935

Immunohistochemistry validation image for anti-Glycine Transporter 1 (GLYT1) (AA 500-600) antibody (ABIN1841935)

A cross section of a mouse retina labelled with ABIN1841935 (rabbit polyclonal anti-glycine transporter 1, GLYT-1 1:400) and a Alexa488 conjugated secondary donkey anti-rabbit antibody 1:400(green). 
ABIN1841935 specifically labels glycinergic amacrine cells in the mouse retina with no unspecific labelling in other retinal layers. Nuclei are labelled with DAPI (blue).

Read the full report

Successfully validated (Immunohistochemistry)
by Kleinlogel lab, Institute of Physiology, University of Bern, Switzerland
No. #100063
Date 11/20/2016

Glycine Transporter 1 (GLYT1) (AA 500-600) antibody

Validation images

Summary with detailed information who validated the product

Detailed Validation Protocol

Image details

Anatomy of a Validation Report

For validated products validation reports are avaibale on

A report contains detailed information about the validation protcol, used methods and results.

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