CUT&RUN Protocol:
Get your free copy!
field marked with (*) are mandatory
Your desktop and mobile views are connected.
Moving desktop elements between page sections will alter mobile and vice versa.
Hold ‘cmd’ or ‘ctrl’ and then drag an element to a new section to avoid changes.
Moving items within sections won’t affect the other view.
Mobile Tip
Think of your mobile view as a condensed summary of desktop.
A. Expand the content tree to the left
B. Select the visibility icon beside the elements you’d like to show or hide.
Hide elements as needed
All page elements should sit inside the blue page guides. Anything outside is highlighted in red.
Layout Assistant helps you get inside the bounds fast.
A. Navigate to Page Properties
B. Under Layout Assistant, click “Entire Page”. Or select a page section, and apply to a section at a time.
Let’s publish (don’t worry it will only be visible to you).
A. Click ‘Save’,
B. Navigate back to the Page Overview screen
C. Click Publish
CUT&RUN: Better than ChIP-seq!
Data taken from: J. Skene & Steven Henikoff (2018), Nature Volume 13
New CUT&RUN Sets for mapping Protein-DNA Interaction
| CUT&RUN Pro Complete ABIN6923135 | ✔ | ✔ | ✔ | ✔ | ✔ | |
| CUT&RUN Pro Direct ABIN6923136 | ✔ | ✔ | ✔ | ✔ | ||
| CUT&RUN Pro Sec ABIN6923137 | ✔ | ✔ | ✔ | ✔ | ||
| CUT&RUN Pro ABIN6923138 |
✔ | ✔ | ✔ | |||
| CUT&RUN Core Complete ABIN6923131 | ✔ | ✔ | ✔ | ✔ | ||
| CUT&RUN Core Direct ABIN6923132 | ✔ | ✔ | ✔ | |||
| CUT&RUN Core Sec ABIN6923133 | ✔ | ✔ | ✔ | |||
| CUT&RUN Core ABIN6923134 |
✔ | ✔ |
Better Data
Only 1/3 of sequencing Reads required
Less Noise
Easier Peak-Calling Higher Reproducibility
Less Sample
10 x less Sample in comparison to ChIP-seq
Obtain purified DNA from Cells within 1 Day
Fast
CUT&RUN: Workflow
Proven Components
First set of matching high quality reagents on the market gives you a worry free access to CUT&RUN.
Consistency
A single source ensures a consistent set of reagents and protocol. Long-term availability and reproducibility guaranteed.
Save Time and Money
Get the CUT&RUN set tailored to your needs and save money compared to purchasing the components separately.
R
M
Our Sets include the following Components:
CUT&RUN is performed on intact cells or nuclei without fragmentation. The cleaved chromatin complexes diffuse out of the nuclei where they can be harvested in supernatant.
An antibody against the protein or histone modification of interest is bound to chromatin in situ. Subsequently, a Protein A-micrococcal nuclease (pA-MNase) or Protein A and Protein G micrococcal nuclease (pAG-MNase) fusion protein is tethered to the Fc region of the antibody. The nuclease then cleaves around the target.
Antigen recognition can either be direct or indirect via a secondary antibody (see figure below).
antibodies-online Inc.
support@antibodies-online.com
www.antibodies-online.com
CUT&RUN offers a new approach to study Protein-DNA interaction. CUT&RUN overcomes various downfalls of ChIP-Seq with improved workflow. Especially the background noise is significantly reduced.
Cleavage Under Targets & Release Using Nuclease
M
R
M
R
Positive and Negative Control Antibodies are important to assess cleavage and chromatin release without the need to sequence the released DNA fragments.
Positive and Negative Control Antibodies
Utilize our Rabbit anti-Mouse Secondary Antibody to ensure efficient tethering of pA/G-MNase in the vicinity of your antigen.
Rabbit anti-Mouse Secondary Antibody
The Rabbit anti-DYKDDDDK Antibody efficiently binds DYKDDDDK-tagged recombinant proteins and allows direct tethering of the pA/G-MNase fusion protein.
Rabbit anti-DYKDDDDK Primary Antibody
The Mouse anti-DYKDDDDK Antibody efficiently binds DYKDDDDK-tagged recombinant proteins and allows indirect tethering of the pA/G-MNase fusion protein in conjunction with the Rabbit anti-Mouse Secondary Antibody.
Mouse anti-DYKDDDDDK Primary Antibody
Concanavalin A coated magnetic beads allow immobilization of living cells without affecting cell physiology while ensuring good sample retention through numerous wash steps to reduce background signal.
Concanavalin A Beads
M
R
Complementary Products available at antibodies-online:
antibodies-online™ Sets
We offer more than 1 million research antibodies, ELISA kits, proteins and related products from over 150 suppliers. This is a unique portfolio on the web.
We help Scientists find the right product
We want to provide transparency in the life science market and simplify the search for the correct product. To achieve this, we constantly work on the search function of antibodies-online.com. By standardizing product data, we make the products on our website comparable. If a scientist has trouble finding the matching product, our scientific support team provides help.
About antibodies-online:
About us
We help Scientists find the right antibody
Benefit from antibodies-online years of expertise and accelerate your research with our CUT&RUN Sets and guidance to find the right antibody.
antibodies-online is the leading vendor-independent marketplace for research reagents, especially antibodies and ELISA kits. The company was founded in 2006 as a spin-off from RWTH Aachen University (Germany). We deliver research products to customers in over 70 different countries. We currently list over 1,95 million research products, which we deliver to universities, biotech and pharmaceutical companies worldwide.
Our diverse team consists of highly motivated biologists, IT- and business specialists. Together we strive to provide scientists with the best possible support for their research.
The successful execution of CUT&RUN hinges on antibody selection. In order to cut adjacent DNA, the pA/G-MNase fusion protein needs to recognize the targeted chromatin part. As is the case with ChIP, the success depends in large part on the affinity of the antibody for its target and its specificity under the conditions used for binding.
antibodies-online GmbH
Schloss-Rahe-Str. 15
52072 Aachen
Germany
Phone +49 (0)241 95 163 153
France
57 rue d'Amsterdam
Paris 75008
France
Phone +33 (0)1 76 63 32 65
United States
PO Box 5201
Limerick, PA 19468
USA
Phone +1 877 302 8632
Germany
CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays
Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays is a fusion of Proteins A and G to Micrococcal Nuclease. The pAG-MNase fusion enzyme may be used in conjunction with any of our CUT&RUN Product Sets.